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The N-terminal tag expression vectors contained a sequence at the translational start site to provide a partial match with the down-stream box (ATG AAT CAC CAT), shown to provide enhancement of translation [ 40] and a decahistidine (H10) tag for enhanced affinity for Nickel resins compared with hexahistidine (H6) tags (data not shown).
It is demonstrated that filling this pocket can confer ligands with dramatically enhanced affinity for Chk1.
Previously, we have engineered an IL-2 "superkine" with enhanced affinity for IL-2Rβ.
This observation suggests that there is an advantage to the molecular modification of DARPin 23.2, an enhanced affinity for CD4.
It may also be due to enhanced affinity for GABA or an increase in the duration of the GABA-gated channel opening [34].
Hydrophobic activated carbons are considered as interesting materials for air or water remediation due to their enhanced affinity for slightly water-soluble organic species.
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It has been suggested that insulin analogs may be associated with an increased risk of cancer compared with human insulin, owing to enhanced affinities for the insulin receptor or the insulin-like growth factor receptor [ 97].
It is postulated that the enhanced affinity of chromium for nitrogen leads to a reduced mobility inside the alloy, thus prohibiting a timely surface passivation.
Increased transferase recruitment can, for instance, be induced by an enhanced affinity of the methyltransferase for the methylated nucleosomes.
The authors explained this effect by enhanced affinity of such NAs for polyvalent substrates.
We solved the crystal structure of UbV.7.2 and rationalized the molecular basis for enhanced affinity and specificity for USP7.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com