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Our design comprised de novo synthetic cancer-specific promoters and, to enhance specificity, an RNA-based AND gate that generates combinatorial immunomodulatory outputs only when both promoters are mutually active.
Furthermore, a melanoma specific promoter was used to enhance specificity of the DNA-delivery strategy.
These proteins likely include phosphoproteins that bind directly to 14-3-3s 14-3-3s 14-3-3sbind indirectly to 14-3-3s as comproteinsof multhatubindt complexes and proteindirectlyave been isolatod 14-3-3s 14-3-3s 14-3-3sh the HT experiments use strasegies to enhancomponentscity).
These features are useful to combat relapse, mitigate over-activation, and enhance specificity.
Combination of these regions synergistically modifies DNA binding to further enhance specificity.
We developed a multiplex PCR system designed to enhance specificity for MAP detection in a single PCR reaction.
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All the mutants show enhanced specificity constants for CB1954, and, apart from N71S, the enhancement is selective for CB1954 over nitrofurazone.
Hox transcription factors can co-operatively bind with PBC-class co-factors, enhancing specificity and affinity for their appropriate binding sites.
Beyond improving affinity, the E-DNA clamp-switch sensor also enhances specificity.
Remarkably, in one sequence context, the DAP modification enhanced specificity against mismatches.
TALENs display enhanced specificity as well as reduced off-target action compared to ZFNs.
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