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These supernatants were derivatized with isobutyl groups to enhance fragmentation for quantitative analysis by tandem mass spectrometry.
Multistage activation (MSA) for neutral losses of 98.0, 49.0, and 32.33 Da was enabled to enhance fragmentation of phosphorylated peptides.
The Rapporteur proposes to extend the scope to offline sales, as he believes that differentiating between online and over the counter sales would further enhance fragmentation of the acquis.
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This derivatization creates a strong acidic group which greatly enhances fragmentation ability of tryptic peptides to produce b and y fragments.
Enhanced fragmentation of SLRPs is evident in degenerate articular cartilage and meniscus.
The partial nuclear breakdown in these double mutants could be due to residual activity in the DNaseII lo hypomorph, or another nuclease like endo G. Mukae et al. (2002) found that dICAD P mutants had a block in nucleosomal fragmentation whereas DNaseII lo mutants had enhanced nucleosomal fragmentation.
In addition to their being proapoptotic, these inhibitors also cause enhanced mitochondrial fragmentation that accompanies a time-dependent loss of optic atrophy 1 (OPA1), suggesting an impairment of mitochondrial fusion.
It has been demonstrated that activated PKCδ phosphorylates DNA-PK and induces its dissociation from DNA, inhibiting DNA repair and enhancing DNA fragmentation [54].
Given that LRRK2 was highly expressed in these diabetic rats, it was not surprising to find that, in our study, LRRK2 elevation enhanced mitochondrial fragmentation.
Conversely, siRNA-mediated p32 knockdown enhanced mitochondrial fragmentation accompanied by a loss of detectable levels of the mitochondrial fusion mediator proteins Mfn (mitofusin) 1 and Mfn2.
It is reported that DAP3 overexpression could promote apoptosis, the downstream mechanism of which may include enhanced mitochondrial fragmentation, decreased mitochondrial membrane potential and increased permeability via opening of transition pores and release of cytochrome c (Berger et al, 2000).
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