Transcriptional enhancers have been used to enhance expression of specific promoters in transgenic plants and in activation tagging studies to help elucidate gene function.
Important class of regulatory elements are enhancers: discrete DNA elements, able to enhance expression of their target genes in a tissue specific fashion.
In addition to pleiotropic enhancers, the ability of TAL1 OSs to enhance expression in the heart could reflect a role in fetal cardiac endothelial cells in hematopoiesis.
An additional ∼800 bp upstream region was able to enhance expression specifically in proboscis, suggesting the existence of enhancer elements.
A parallel group of rVV was constructed to include the heterologous eukaryotic tissue plasminogen activator (tPA) signal sequence to assess if this would enhance expression and immunogenicity.
Since combination of small chemical compounds with diverse targets highly promote cell reprogramming in vitro, it is very interesting to know whether these cocktails also enhance expression of pluripotent genes in this process.
It is also noteworthy that in tumor cells NRF2 can enhance expression of genes involved in PPP such as glucose-6-phosphate dehydrogenase, phosphogluconate dehydrogenase, transketolase, and transaldolase 1, which contribute to NADPH regeneration [92].
We have additionally identified putative regulatory elements that may enhance expression of transgenic silks.
A murine genomic sequence equivalent to −10 has been shown to enhance expression of murine SCL in erythroid cells [33].
Protein-coding sequences are commonly re-designed to enhance expression, but there are no experimentally supported design principles.
The histone deacetlyase inhibitor sodium butyrate has been demonstrated to synergistically enhance expression when used with tetracycline-regulated systems [21].
