However, the myristoylation deficient G2A mutant (Fig. 4B, lane 5) and CA deletion mutant (ΔCA) (Fig. 4B, lane 6) were unable to enhance budding of co-expressed PRE-dependent HIV-1 Gag-GFP compared to co-expression of Rev-dependent wild type HIV-1 Gag (Fig. 4B, lane 2).
CLE42 is expressed strongly in shoot apical meristem (SAM) and axillary meristems to enhance axillary bud formation (Yaginuma et al., 2011).
Recently, TDIF and CLE42 peptide were found to have an in vivo activity to enhance axillary bud formation and there are indications that PXY/TDR is involved in this process [ 19].
Our study found that up-regulated expression of OsNPF7.2 significantly enhanced tiller bud growth, and that down-regulated expression of OsNPF7.2 impaired tiller bud development (Fig. 2), suggesting that the enhanced translocation of nitrate into leaf sheath in OX lines might contribute to faster growth of tiller bud determining rice tiller number.
In particular, the softer gel enhances the bud expansion and cleft formation of SMG, whereas the stiffer gel attenuates them.
As an evidence, a novel function in enhancing axillary bud formation has been recently disclosed for Arabidopsis TDIF peptide [ 44].
The auxin signalling mutation axr1-3, which does not affect axillary shoot formation in a wild-type background [ 66], enhanced axillary bud loss in combination with rps10b-1.
Additionally, the fact that cysts contact enhances budding recalls the mechanisms like ligand-receptor interaction might be involved in the process as it happens in other systems [ 8].
SlAGO4C and SlAGO15 could not be detected in any collected stages and SlAGO5 displayed gradually enhanced expression during bud development, SlAGO7 could be detected on stage 4 and stage 5 of flower buds only.
We have shown that CaZF can enhance hyperosmotic stress tolerance in budding yeast and it can partially suppress the osmosensitive phenotype of the mutants lacking Hog1p and/or Calcineurin activities.
