By analyzing the single-molecule properties of engineered motors, we demonstrate that the non-catalytic domain has a key role in the motility mechanism by acting as a 'foothold' that allows Kar3 to bias translocation towards the minus end.
Using a series of in vitro and in vivo studies with orthotopic and genetically engineered mouse models, we demonstrate the mechanisms by which EGFL6 stimulates tumor angiogenesis.
Applying our engineered lentiviral vectors, we demonstrate that vector silencing typically occurs prior to or simultaneously with the induction of an Oct4-EGFP pluripotency marker.
Using genetically engineered tracing mice, we demonstrate that, in dystrophic muscle, specialized cells of muscular, endothelial, and hematopoietic origins gain plasticity toward a fibrogenic fate via a TGFβ-mediated pathway.
By using both human breast cancer cells and genetically engineered mice, we demonstrated that RBP2 is critical for breast cancer metastasis to the lung in multiple in vivo models.
When combined with the temperature-moderating characteristics of engineered phase change materials (EPCM), we demonstrate that an engineered exothermal chemical heating unit can produce a consistent constant-temperature incubator for isothermal NA amplification suitable for a variety of isothermal techniques.
Using CRISPR/Cas9 technology to engineer precise genetic deletions, we demonstrate that poised enhancers are necessary for the induction of major anterior neural regulators.
Using an engineered transcriptional repressor gradient, we demonstrate that overlapping repressor and activator binding sites provide more robust repression and sharper expression boundaries than non-overlapping sites.
In this work, using the non-electrospinning Spinneret based Tunable Engineered Parameters (STEP) platform, we demonstrate fabrication of long (mm-cm) aligned polystyrene (PS) nano and microscale tubes in single and multiple layers.
Using an integrin-binding fibronectin fragment as a model protein, we demonstrate that engineered knob protein fusions bind consistently and specifically to fibrin ogen).
