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Genetic engineering of viruses, for peaceful research, has become routine and standardized.
The engineering of viruses by insertion of a tissue-specific miRNA recognition element (MRE) into viral mRNA can restrict viral tissue tropism.
Engineering of viruses to express novel genes have also led to the use of these vectors as a novel form of gene therapy.
Furthermore, through genetic engineering of viruses, new colloids have been created that are interesting to materials science for their potential to form self-assembling functional materials, such as biosensors and transducers.
This well-studied genus has led to many discoveries within the field of virology including developments of biological assays using local lesion hosts, virus purification and crystallization, visualization under the electron microscope, identification of the first viral movement protein, and the engineering of viruses to express foreign genes in plants.
The lack of icosahedral symmetry and modular organization in the genome of these viruses has facilitated engineering of viruses expressing fluorescent proteins, and these fluorescent proteins have provided important insights about the molecular and cellular basis of tissue tropism and pathogenesis.
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These results suggest an approach to the engineering of virus resistance.
Our results demonstrated successful rational modification of AAV2 as a targeting delivery vehicle, establishing a facile platform for precision engineering of virus-based nanoparticles in basic research and therapeutic applications.
The engineering of artificial viruses by dismantling virus particles or incorporating viral features into nonviral vectors represents a novel strategy to combine "the best of both worlds".
An infectious FAdV-9 genomic clone, constructed by homologous recombination in Escherichia coli, was used for engineering of recombinant viruses.
Genetic engineering of phage viruses provides opportunities for building novel bio-nanomaterials by integrating biology, chemistry, physics, materials science, and electric engineering.
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