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The protocol combines PCR-based synthesis and engineering of translation templates with a combined transcription translation system.
Alternatively, biophysical models have been developed to predict and guide engineering of translation initiation signals in bacteria, thereby providing an additional point of control.
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By establishing a design strategy suitable for biomedical applications, we have provided a platform for the engineering of sophisticated, translation-ready DNA nanodevices.
Realizing the full potential of pig genetic engineering requires translation of the complete repertoire of genetic tools currently employed in smaller model organisms to practical use in pigs.
With the superior bioprinting speed, flexibility and scalability, this new prevascularization approach can be broadly applicable to the engineering and translation of various functional tissues.
We anticipate that improved knowledge of how exit tunnel proteins contribute to protein expression and folding could be key in the context of rationally engineering bacterial translation for efficient production of active, structurally complex proteins.
Recent advances in regenerative medicine, including novel approaches to urological tissue engineering, have accelerated the process of translation of cell-based therapy from the bench to the clinic.
Major, expanding areas include "business, computer science, [and] medicine …." There is also "a great deal of translation in the engineering field".
These perspectives are intended to lay the foundation for an improved understanding of interlinked mechanisms controlling transfection and toxicity and their translation for improved engineering of polycation-based transfectants.
Recent regenerative medicine and tissue engineering strategies for bone and cartilage repair have led to fascinating progress of translation from basic research to clinical applications.
Integration of all available data led to reverse engineering of the published TCZ model and its translation to OKZ in the form of three potential PK/PD model parameterizations (Supplementary Table S1).
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