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Reduced expression could be overcome by adjusting the composition of the CDM or rational engineering of the expression host.
The engineering of the expression vector and the expression of N-labeled proteins in Escherichia coli were as described previously (19).
Strategies to improve (specific) production titers include genetic engineering of the expression vectors and host cell line combined with optimization of cultivation and feeding strategies (Kim et al. 2012).
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Recent proof-of-concept studies highlight the potential of plastid genome engineering for the expression of resistance traits, the production of biopharmaceuticals and metabolic pathway engineering in plants.
2A functions in all the eukaryotic systems tested to date and has already been applied, with great success, to a broad range of biotechnological applications: from plant metabolome engineering to the expression of T-cell receptor complexes, monoclonal antibodies or heterodimeric cytokines in animals.
Moreover, a more detailed study of the effects of parameter modification (rate constants and kinetic order) indicated that genetic engineering of the hydrogenase expression, activity and stability may lead to increased performance of the process.
However, no data on the engineering of its expression and the effect on cellulase gene expression have yet been reported.
Real-time quantification of recombinant proteins is important in studies on fermentation engineering, cell engineering, etc. Measurement of the expression level of heterologous proteins in bacterial fermentation broth has traditionally relied on time-consuming and labor-intensive procedures, such as polyacrylamide gel electrophoresis, immunoblot analysis, and biological activity assays.
In addition the transcription control elements of the genes can be used for molecular genetic engineering, targeting the expression of proteins that can be used to manipulate neuronal activity.
The development of genetic engineering has enabled the expression of HLZ in microorganisms [20], eukaryotic cells [21] and plants [22].
Transformation of the plastid genome has a number of inherent advantages for the engineering of gene expression in plants.
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