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Thus far, metabolic engineering has been largely restricted to the deletion and/or massive overexpression of genes involved in byproduct formation or in the rate determining steps of a metabolic pathway.
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The creation of 1 nm size nanogold particles by a materials engineering process has been largely undeveloped.
Intercellular chemical communication is commonly exploited for the engineering of living cells but has been largely ignored by efforts to build artificial cells.
With the rapid development of genetic tools in recent years, the intrinsic intractability of clostridia has been largely overcome, thus, engineering clostridia for production of chemicals and fuels has attracted increasing interests.
Perhaps for historical reasons, namely the initial string of transcription network papers, the field has been largely dominated by engineering transcription networks.
This has been largely accomplished by applying engineering principles.
The insurgency has been largely vanquished.
The experience has been largely positive.
But this has been largely ineffective.
(The book has been largely discredited).
But the response has been largely positive.
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