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Moreover, DNA can be used as a promising material for nanobiotechnology, and the nanostructures of DNA can be engineered to recognize a variety of molecules.
Because the zinc finger domain can be engineered to recognize a wide range of novel DNA sequences, a specific ZFN can be designed, produced, introduced and directed to a specific genomic locus for cleavage.
To overcome this limitation, T cells can be isolated from the peripheral blood of cancer patients, genetically engineered to recognize a specific tumor-associated antigen, expanded in vitro, and adoptively transferred back to the patient.
ZFNs, chimeric fusions between a zinc-finger DNA binding domain and the FokI nuclease domain, have the ability to recognize and cut existing sites in a genome because the zinc-finger domain can be engineered to recognize a variety of different DNA sequences.
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When engineering NR, the DNA binding domain (DBD) of these receptors can be engineered to recognize an artificial promoter containing multimeric-binding sites and a minimal promoter [ 1, 2, 21].
Because trastuzumab is a mAb engineered to recognize the extracellular domain of the human HER2 receptor, it does not recognize the mouse homolog, neu [ 16].
We report crystal structures of a binding protein engineered to recognize the agonist serotonin and the antagonist granisetron with affinities comparable to the 5-HT3 receptor.
The crystal structures of a binding protein engineered to recognize serotonin (5-HT) and the anti-emetic granisetron with affinities comparable to the 5-HT3 revealor reveal important structural details of ligand recognition in the 5-HT3 receptor.
We will review our previous work that describes the utility of an antibody fragment (Fab) engineered to recognize 8-oxoG in DNA.
The aminoacyl-tRNA synthetase (RS) is engineered to recognize unnatural amino acids by mutating the active site, based on a known structure [6], [7].
ZFNs are artificial restriction endonucleases consisting of a non-specific nuclease domain fused to a zinc finger array which can be engineered to recognize specific DNA sequences of interest.
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