To investigate the fate of human prostate epithelial cells, when engineered to maintain expression of clusterin protein, we have used an IRES-hyg vector and hygromycin selection.
The tribe could even form a company to serve as an Internet service provider and employ wireless engineers to maintain the local network.
Provinces deep in the interior have few skilled workers or engineers to maintain the automated gear that keeps mirrors focused on towers that transfer the heat from sunbeams into fluids.
The engineers — attempting to maintain the spirit of collaboration — tried twisting the Japanese cable more tightly, in the hope that it would perform better.
Mining engineers first tried to maintain the supports by pumping in saltwater, intending to raise the salinity of the floodwater to the saturation point before the structure collapsed, but that did not work.
A single company supplies 90% of raw vinyl for American records, leading to daily supply shortages at the 15 remaining pressing-plants; only a handful of engineers know how to maintain the old equipment.
Stably transformed T. gondii expressing the dsRNA, which was capable of growing in the presence of the prodrug 5-fluoro-2′-deoxyuridine (FDUR), appeared to maintain the engineered plasmid as an extra-chromosomal DNA.
Because the encoding region possesses stop codons, repeat sequences and secondary structure, we hypothesized that genetically engineered E. coli are required to maintain the number of purine-pyrimidine repeats and reading frame when producing expression plasmids containing the Mig-7 sequence.
Throughout all experiments, cultures and biofilms were grown at 30°C in M9-AADO (Amino Acid Drop Out) medium without lysine, containing 50 µg ml−1 kanamycin and 20 µg ml−1 tetracycline to maintain the engineered and the marker plasmids, respectively [34].
Found it impossible either to extinguish fire or feather prop, and had to have rudder tied by engineer to maintain straight flight, as the rudder trimmers had been shot away.
