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A non-infectious simulant was designed and engineered to include the nucleic acid signature of VEEV (Venezuelan Equine Encephalitis virus), Influenza virus, Rift Valley Fever virus, Machupo virus, Lassa virus, Yellow Fever virus, Ebola virus, Eastern Equine Encephalitis virus, Junin virus, Marburg virus, Dengue virus, and Crimean-Congo virus, all in a single construct.
A PCR amplicon was engineered to include the MET25 promoter and ~50 bp of flanking sequence on each end.
The gene-specific forward primers were engineered to include the g10 element sequence while the reverse primers had the rpsT element.
The 5' external primer was engineered to include the sequence of the TEV enzymatic cleave site and the 3' primer contained the NotI restriction site.
The peptide was engineered to include the C-terminal sequence VFPLECHHHHHH, a hexahistidine tag for site-specific radiolabeling with 99mTc(CO)3(OH2)3]+ (99mTc-Tricarbonal) (20).
The same targeted genomic region was engineered to include the FRT-Neo-FRT-loxP cassette at the position identical to that in the Cbfb f allele, using recombineering technology.
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Many gene expression systems have also been engineered to include various parts of the SV40 genome, such as its promoters, splicing signals, and poly(A) cleavage signals.
Fibronectin-like protein polymer has been engineered to include 13 copies of the RGD-rich sequence, resulting in a protein with a stable three-dimensional conformation and molecular mass of 110 kDa.
After conjugating the TfRscFv-GAL4 to the plasmid GAL4rec-pGes which was engineered to include a specific long sequence in the upstream activating sequence (5'-cggrnnrcynyncnccg-3', GAL4rec) and reporter gene GFP, protein-DNA complex incubated with hepatic carcinoma cells HepG2, and the GFP expression in cells was detected by fluorescence microscopy.
In addition, the protein was engineered to include an extramembrane C-terminal extension for crystal contact formation.
The E1α sequence was engineered to include an N-terminal His-tag.
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