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B. sacchari mutant (unable to produce polymer) was engineered to host PHA biosynthesis genes (phaPCJ) from Aeromonas sp. In addition, a two-step bioprocess to increase biopolymer production was developed.
Recently, the effect of single chr 21 genes on the trisomic trancriptome was established by comparing the genome-wide expression from mouse ES cells, engineered to host the whole human chr 21, with those overexpressing only single chr 21 genes.
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Nonetheless, these studies clearly demonstrate the advantage of using a pathogenic fungus engineered to secrete host cytokines in order to achieve a biological "print out" of those factors that mediate protective host immunity.
Already, the host's career moves -- like her decision to be a judge on "American Idol," an enormous promotional platform -- have seemingly been engineered to enhance her daytime host identity.
This bacterium was extensively engineered to create a suitable host for polyketide production.
This exemplifies the nature of a host engineered to produce a compound it does not normally produce, showing that a variety of the pathways are not evolved.
Moreover, the proteomic analysis identified a list of potential target genes/proteins which can be engineered to generate stress-resistant hosts for high efficiency production of hexane [ 71, 72].
Yeast cells such as Pichia pastoris may provide much faster and cheaper ways of production (Çelik and Çalık 2012); while this yeast can be engineered to serve as a suitable hosts, highly complex proteins such as antibodies can be difficult to express efficiently in this system (Nielsen 2013).
S. coelicolor M512 (Δ redD Δ actII-orf4 SCP2− SCP2−) (32), S. coelicolor M1152 [Δ act Δ red Δ cpk Δ cda rpoB(C1298T ], and S. coelicolor M1154 [Δ act Δ red Δ cpk Δ cda rpoB(C1298T) rpsL(A262G)] (33) have all been engineered to be good heterologous expression hosts for secondary metabolite pathways.
Comparable positive effects were obtained when the host bacteria were engineered to overexpress the gene products of otsA and otsB, the two enzymes involved in trehalose synthesis.
Recombinant host strains were engineered to express genes for three bacteriocins, enterocin A, hiracin JM79 and enterocin P, each with potent antimicrobial activity against E. faecalis.
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