Sentence examples for engineered terminal from inspiring English sources

We discovered that template variations, including engineered terminal truncations, have significant influence on the position of the crossovers in the recombination-dependent PCR.

In the present study, an extremophilic GH11 xylanase was stabilized by an engineered N-terminal disulphide bridge.

We report a simple and versatile approach for the conjugation of luminescent CdSe ZnS core-shell quantum dots (QDs) to proteins through coordination of engineered C-terminal oligohistidine sequences.

A flexible, trifunctional poly ethylene glycol -succinamide-Lysine-Lysine-maleimide (PEglycol -succinamide-Lysine-Lysine-maleimidemultaneously allow biotin taglycol -succinamide-Lysine-Lysine-maleimide integlycol -succinamide-Lysine-Lysine-maleimideeglycol -succinamide-Lysine-Lysine-maleimideed Fc fragment with an engineered C-terminal selenocysteine residue.

Accordingly, we have developed a single-chain variable region fragment (scFv) of J591 with an engineered C-terminal Cys residue (henceforth referred to as J591c-scFv) for conjugation to a bifunctional chelator (Fig. 1).

Ylera et al. [27] selected aptamers against Aβ40 coupled to a Sepharose column through an engineered N-terminal cysteine.

For subsequent scale-up experiments the MCMJR1 gene was transferred to a variant of pET-28b for expression of a genetically engineered N-terminal fusion with the small ubiquitin modifying protein (SUMO; [47]), preceded by a His6-tag.

Chimeric segments from previously described plasmids pGB/I-II-IIIHC, pGB/II-IIIHC, and pGB/IIIHC that contain genome-length GBV-B cDNA with chimeric GBV-B/HCV 5'NTRs [3] were PCR-amplified using a sense primer hybridizing upstream of the T7 RNA polymerase promoter and an anti-sense primer hybridizing to the appropriate GBV-B core codons followed by an engineered 3'-terminal AscI restriction site.

Proteins purified from Sf9 cells were expressed using the pFastBac1 vector (Life Technologies) with an engineered N-terminal Flag-HA tag.

In order to provide the possibility to render the leader peptide traceless, a Factor Xa protease cleavage site was engineered N-terminal to the random peptide sequence.

To mimic the native membrane insertion topology, we biotinylated proteins enzymatically at an engineered C-terminal tag (Aricescu et al, 2006) and attached them via the biotin label to streptavidin that was covalently coupled to the Biacore chip surface (O'Callaghan et al, 1999).