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The GABA titer here was somewhat lower than that of GADΔpknG (Okai et al. 2014) and recombinant C. glutamicum strain harbouring pHGmut (Choi et al. 2015) which expressed E. coli GAD and much lower than that of GABA6C and GABA6F (Jorge et al. 2017) whose putrescine pathway was engineered (Table 3).
Briefly, PCR products were generated using primers into which restriction endonuclease sites were engineered (Table 1).
Many mouse models with varying degrees of bowel aganglionosis, hypoganglionosis, and other defects have been isolated or engineered (Table 1); many of these are caused by disruption of orthologs of human HSCR genes.
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In all three field stations, applicators experienced the greatest amount of BuChE inhibition, followed by technicians and engineers (Table 2).
Interestingly, propionate was also concomitantly produced with 1-propanol in our metabolically engineered strains (Tables 1 and 2).
The method has been used to genetically engineer the table grape varieties Silcora and Thompson Seedless.
Turner had the Ford engineers' tables and charts showing they constantly juggled the size and air-pressure of the tires in hopes of making the prototype Explorer more stable.
The resulting engineered strain FZBHarpin (Table 4) contained two copies of the hpa1 gene and the antibiotic marker fragments (lox-Km, lox-Spec, Cre-lox).
In our studies, we used the eGFP sequence as a reporter, whose initiator AUG is located 12 bases downstream of the engineered HindIII site (Table 1).
The native FtsZ gene contained a total of 19 rare codons (1.58% of all codons) which were eliminated in the engineered gene (see Table 2), however NS5B contained a significantly larger number of rare codons (62 or 3.46% of all codons) but elimination of rare codons from NS5B had no effect on the level of expression.
Selected genomic regions targeted by sgp53-1 and sgp53-3 were amplified using barcoded primers with engineered adaptor regions (Table 1) and Phusion HiFi polymerase (NEB; Beverly, MA, USA) using five cycles at an annealing temperature of 56 °C followed by 20 cycles at an annealing temperature of 68 °C.
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