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The physiology of differentiated neurons within tissue engineered sheets was evaluated.
Tissue engineered sheets containing laminin and/or heparan sulfate had a balanced expression of contractile and relaxant motor neurons.
Accordingly, the method of preparing living tissue engineered sheets was modified to obtain an optimal alignment of SMC before rolling them into a tubular form.
An enriched nitrergic neuronal population, evidenced by an increased expression of neuronal nitric oxide synthase, was obtained in tissue engineered sheets that included collagen IV.
The smooth muscle component of the tissue engineered sheets maintained a contractile phenotype irrespective of the ECM composition, and generated equivalent contractions in response to potassium chloride stimulation, similar to native intestinal tissue.
These data implicated the potential of this method for the preparation of genetically engineered ASC sheet and future MI treatment.
Once placed, the medium was dropped onto the center of the sheet to spread folded parts of the transferred engineered cardiomyocyte sheets.
We present the development of a novel lung air leak sealant using tissue engineered cell sheets.
This modification was also successfully performed on dispersed hepatocytes and engineered hepatocyte sheets.
Here we describe a subcutaneous human liver construction allowing for rapidly vascularized grafts by transplanting engineered cellular sheets consisting of human primary hepatocytes adhered onto a fibroblast layer.
In this study, we hypothesized that the ECM could be used to induce MSC differentiation to SMCs for engineered cell-sheet construction.
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