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We developed a genome-scale library of ATFs that display an engineered interaction domain (ID) to enable cooperative assembly and synergistic gene expression at targeted sites.
We anticipate that such promising outcome can be further improved by employing different morphologies of FeSe2 and engineered interaction with other conductive supports suitable for their applications in electrochemical sensing and energy conversion.
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In this review, we focus on recent advances in NP-biomacromolecule interactions, highlighting the control of biomacromolecule structure and function through engineered interactions with NP surfaces.
These results allow for the rational design of more potent Top1 inhibitors through engineered interactions with as yet unutilized Top1 active-site residues including: Glu356, Asn430, and Lys751.
However, the parameters of this developing technology have yet to be defined and it has not yet been determined if these modified vectors actually infect cells via these engineered interactions.
This capability will allow synthetic biologists to monitor and even engineer interactions with host networks, which could become critical as the field moves to engineer larger and more sophisticated genetic networks.
For example, Martin et al. have successfully expressed enzymes from plants, yeast and Escherichia coli to produce amorphadine, a precursor to an anti-malarial drug artemisinin [ 19] and Anderson et al. have engineered the interaction between bacteria and cancer cells to depend on heterologous environmental signals [ 20].
Engineered receptor RBP interactions are also useful in phage applications such as phage display or bacterial detection.
Our studies suggest that effectively quenching the conformational dynamics of an intein through engineered allosteric interactions could deactivate intein splicing or cleaving.
Engineered domain peptide interactions have also been used to design kinase sensors 36, 37, 38, 39 using a general strategy where two different fluorescent domains are joined by an intermediate section containing a peptide substrate for a kinase of interest a flexible linker and phospho-binding domain (e.g. SH2 or 14-3-3 14-3-3 14-3-3
"If you engineer an interaction, it won't work," Tadigadapa says.
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