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A Modoc/yellow fever chimeric virus was engineered in which the structural prM and E genes of yellow fever virus 17D were replaced by the homologous genes of Modoc virus.
To this end, a construct encoding the invariant chain (Ii) was engineered in which the Class II associated invariant chain peptide (CLIP) sequence was replaced by an immunogenic epitope derived form Heat Shock Protein 60, HSP60 178–186.
Next, a tricomponent complex was engineered in which the three molecular entities (the D3 antigen, COMP coiled-coil domain, and Z domain) were genetically connected in tandem to create the D3 COMP Z tricomponent complex, or its reversal oriented construct, Z COMP D3.
A second construct was also engineered in which the stop codon was altered by a single base pair mutation of TGA to GGA yielding a glycine residue substitution.
A series of mutant carriers were engineered in which the interaction energy of the cytoplasmic network increased or decreased compared to the wild-type.
A chimeric cytokine receptor named 4αβ was engineered in which the IL-4 receptor α (IL-4Rα) ectodomain was fused to the shared βc subunit, used by IL-2/IL-15.
Similar(54)
In the laboratory, disarmed strains of Agrobacterium can be engineered in which most of the T-DNA segment of the plasmid is replaced with one or more foreign genes of interest, comprising a novel T-DNA.
Particular attention is given to engineered landscapes in which the direct anthropic alteration of processes is significant.
The solution for plasma facing materials likely consists of engineered structures in which the layer of plasma facing material (PFM) is integrated with an engineered structure that cools the PFM and may also transition with graded composition.
We have studied genetically engineered mice in which the fbxw7 gene is conditionally knocked-out in the intestine (fbxw7ΔG).
The role of wiring molecules in circuit assembly is tested directly in genetically engineered animals, in which the corresponding gene has been selectively mutated.
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