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Exposure of GPC3+ HCC cells to this engineered cell line resulted in significant in vitro cytotoxicity and cytokine production.
By using southern blot and fluorescence in situ hybridization (FISH), a candidate engineered cell line, number 37, was chosen.
The resulting cells produced 2-fold more lactate than a baseline engineered cell line, representing the highest photosynthetically generated productivity to date.
For example, you may have some lung cells in a culture flask, but are they primary cells, cancer cells, or an engineered cell line?
This genetically engineered cell line is a valuable tool for the fish research community especially in research areas investigating the biological function of viral proteins.
An engineered cell line was constructed to further study TbPP2A-1 and to facilitate the discovery of other cell cycle regulatory genes.
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ADCC assays are commonly performed using peripheral blood mononuclear cells (PBMCs), natural killer (NK) cells or engineered cell lines as effector cells.
Finally, to facilitate rapid dissemination of data to the larger scientific community, a shared database and a public 4DN data browser will be established which includes all data, detailed protocols, engineered cell lines and reagents used across the Network.
Fluorescence microscopy of genetically engineered cell lines in culture can be used to visualise the progression of these cells through the cell cycle, including distinctly identifiable sequential stages of cell division (mitotic phases).
New/improved tests have to be established and validated, considering the use of (metabolically competent) primary (skin) cells, 3D skin models and cells with defined capacity for metabolic activation (e.g. genetically engineered cell lines).
In recent years, progress has been made in BAL regarding genetically engineered cell lines, immortalized human hepatocytes, methods for preserving the phenotype of primary human hepatocytes, and other functional hepatocytes derived from stem cells.
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