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Once this is achieved, the entire repertoire of yeast genetics is available to probe the function of the gene, or to engineer the expression in useful ways.
This natural ability of anaerobic bacteria for tumor colonization has encouraged research aimed to engineer the expression of therapeutic proteins within the tumor (e.g., cytotoxins, pro-drug converting enzymes) as well as gene reporters for in vivo tumor diagnosis (e.g., bioluminescence, magnetic resonance imaging).
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I outline four themes that characterize complex systems in biogeomorphology: multiple causality and the concept of recursivity, the influence of organisms that function as ecosystem engineers, the expression of an ecological topology, and ecological memory.
In the present study we engineered the expression of IL-10 in human adipose-derived mesenchymal stem cells (Adi-IL-10-MSCs) and transplanted these cells early in the disease course to mice with EAE.
To achieve combined cell-type-specific transcriptional targeting and inducible expression, we have engineered the expression of the tetracycline-dependent transcriptional elements (1) to be under the transcriptional control of either the astrocyte-specific, glial fibrillary acidic protein (GFAP) (2) or the neuronal specific enolase (NSE) promoter (3) within a dual adenoviral vector system.
One way to more successfully express genes in metagenomic library hosts may be to engineer the host expression machinery on the basis of the expected prevalence of genes from source hosts.
Pichia pastoris cells transformed with a plasmid engineered for the expression of tilapia growth hormone as a secreted product produced a proteolytically cleaved form of the recombinant protein.
Cleavage of the TRX tag from AtGA3OX4 was enabled by a thrombin site engineered into the expression construct – the Factor Xa site encoded by pET32 failed to cleave.
The motifs derived from osteopontin (SVVYGLRGSGSGS) and BMP-2 (KIPKASSVPTELSAISTLYL) were engineered into the expression construct using a synthetic oligonuleotide approach.
E. coli was also engineered for the expression of a recombinant gonadotropin-releasing hormone (GnRH) vaccine that could be used as a vaccine for the control of fertility and hormone-dependent diseases [ 55].
The effect of 2-aminofluorene (2-AF) on the toxicity of 2-aminoanthracene (2-AA) and 1,6-dinitropyrene (1,6-DNP) was studied in N-acetyltransferase-proficient V79-NHr1A2 cells genetically engineered for the expression of cytochrome P4501A2, and in wild-type V79-NH cells.
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