Sentence examples for engineer cell lines from inspiring English sources
To engineer cell lines, primers were designed to amplify cDNA from the pEF6-HA-pTGFβ1(a) vector for cloning into the multicloning site of the pBMN-Ires-eGFP retroviral vector (courtesy of Dr. Gary Nolan).
In the present work, a handful of genes were investigated separately, but the potential of this approach (using genomics tools to engineer cell lines) may rely on being able to modulate the expression of many genes simultaneously, rather than individual genes.
ADCC assays are commonly performed using peripheral blood mononuclear cells (PBMCs), natural killer (NK) cells or engineered cell lines as effector cells.
Finally, to facilitate rapid dissemination of data to the larger scientific community, a shared database and a public 4DN data browser will be established which includes all data, detailed protocols, engineered cell lines and reagents used across the Network.
Fluorescence microscopy of genetically engineered cell lines in culture can be used to visualise the progression of these cells through the cell cycle, including distinctly identifiable sequential stages of cell division (mitotic phases).
New/improved tests have to be established and validated, considering the use of (metabolically competent) primary (skin) cells, 3D skin models and cells with defined capacity for metabolic activation (e.g. genetically engineered cell lines).
In recent years, progress has been made in BAL regarding genetically engineered cell lines, immortalized human hepatocytes, methods for preserving the phenotype of primary human hepatocytes, and other functional hepatocytes derived from stem cells.
To identify variants with increased affinity to FcγRIIIa, we developed quantitative assays using soluble receptors as well as engineered cell lines expressing FcγRI or FcγRIIIa on the cell surface.
As an example of advanced testing in the field of metabolism in an industrial environment, the introduction of some novel approaches, including the use of genetically engineered cell lines for assessing CYP 2D6-related polymorphic effects is illustrated.
Target cells included PBMC and genetically engineered cell lines in either single- or multiple-cycle infection format.
Created from engineered cell lines, cfDNA Reference Standards are available in both singleplex and multiplex format with cancer-relevant mutations.
