Besides monitoring conformational changes within the unfoldase, these measurements will also help to follow the path of substrate molecules during initial binding, engagement, unfolding, and translocation.
The latter serves as a pivot around which the lid complex rotates in accord with substrate engagement, unfolding, and translocation (Matyskiela et al., 2013; Unverdorben et al., 2014).
The battle unfolded badly.
The rate of engagement and unfolding by the 19S complex relative to the rate of trimming the poly-ubiquitin chain is probably a critical factor in determining the fate of a particular substrate.
The months following the training were nothing short of watching the engagement miracle unfold: The Health Care Institute (HCI) became the catalyst for renewing our staff and engaging the families that they work with.
But soon after their engagement, the truth began to unfold.
Our goals are to decipher the fundamental principles that govern substrate engagement, de-ubiquitylation, unfolding, and translocation by the proteasome.
It welcomes theoretical interventions, interpretations, and engagements with political events, institutions, cultures, and issues as they unfold.
The intent is to allow such PCOI experiences to unfold and enable rich opportunities for discussions and engagements to take place.
As the investigation into the water crisis in Flint, Mich., continues to unfold, disturbing reports have arisen that raise questions about the integrity of government science agencies and their possible engagement in scientific misconduct or even outright science denial.
