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"It's my view it's too absolute, too harsh, and not particularly well enforced over the years.
Internal Turmoil Effectively, as Mr. Assange pursues his fugitive's life, his leadership is enforced over the Internet.
True, rules were so sketchily enforced over the last several years that even minimally increased efforts have become noticeable.
Currently, users with pseudonyms can still post, but analysts say they expect the rule to be slowly enforced over the coming months.
N.B.A. Commissioner David Stern recently said that he would like to see that rule enforced over the full 48 minutes, but the league's competition committee decided otherwise.
In my family we're even willing to give up a little individual freedom for the collective good, heeding a roster of rules enforced over the years by my father.
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Conversely, enforced over-expression of FXYD6 in SMMC7721 cells (Fig. 2E) increased migration and proliferation of these cells, compared with control transfectants of empty vector (Fig. 2F and 2G).
These effects were reverted by enforced over-expression of a murine Ezh2, highlighting an EZH2-specific effect.
This is thought to reflect a primary role for eIF4E in tumorigenesis, since enforced over-expression of eIF4E in vitro causes malignant transformation and deregulated cell growth.
The effect of HRES-1/Rab4 on the accumulation of LC3-II was not affected by rapamycin (see online supplementary figure S2A), indicating that the stimulation of autophagy by genetically enforced over-expression of HRES-1/Rab4 was not dependent on mTOR.
Consistently, COX-2 is abundantly expressed in breast cancer tissue [ 18] and its enforced over-expression in mammary gland epithelia of transgenic mice results in breast tumour development [ 19], suggesting that COX-2 might be an interesting therapeutic target in breast cancer.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com