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In mice, xenotransplants of colon cancer cells with enforced CAR expression formed significantly smaller tumours, whereas CAR inhibition increased the formation of liver metastases.
Enforced CAR expression led to lower caspase 3/7 activity in DLD1 cells upon treatment with TRAIL, whereas no changes were found in HCT116 and SW620, and an increase in the SW480 cell line was found (data not shown).
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In cancer cell lines, CAR inhibition resulted in increased proliferation, whereas enforced ectopic CAR expression led to opposite results.
These experiments showed that RNAi-mediated CAR knock down resulted in increased proliferation, migration, and invasion of gastric cancer cell lines, whereas enforced ectopic CAR expression led to opposite effects.
Dozens of drive-through bottle shops throughout the Northern Territory have enforced a No Car, No Cask policy for years, only to find problem drinkers borrow cars to purchase booze.
In interviews, men have said they would stop if they knew there would be consequences: if a family member or boss would find out, if meaningful fines were enforced, if their cars would be impounded.
Speed limits are strictly enforced by unmarked cars with speed cameras (see flashing of lights later).
It stopped several cars and enforced the 11 p.m. curfew.
IN Lisa Del Papa's childhood, vacations meant long hours of enforced intimacy in the family car.
The driving restrictions on privately owned cars will be enforced in two stages.
For example, a law in Georgia that would have enforced tint laws only on cars registered in the state was thrown out in court as discriminatory against residents.
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CEO of Professional Science Editing for Scientists @ prosciediting.com