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W = {w1, w2 ⋯ wn} is the initial energy set of the sensor nodes.
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High-energy collision dissociation (HCD) of the MS/MS was used to select peptides with a normalized collision energy setting of 27.0; ion fragments were detected in the Orbitrap at a resolution of 17,500.
The ten most abundant ions from MS analysis were selected for further MS/MS analysis using a normalized collision energy setting of 35%.
Each full MS scan (m/z 400 2000) was followed by collision-induced MS/MS scans (normalized collision energy setting of 35%) for the 10 most abundant ions.
The ten most intense peptide ions with charge states ≥2 were sequentially isolated to a target value of 5e3 and fragmented by low-energy collision-induced dissociation (CID) with a normalized collision energy setting of 35%.
The ten most intensive precursor ions were dynamically selected in the order of highest intensity to lowest intensity and subjected to collision-induced dissociation using a normalized collision energy setting of 35% and a dynamic exclusion duration of 1 min. The heated capillary was maintained at 200°C, while the ESI voltage was kept at 2.2 kV.
Three countershocks were initially administered at an energy setting of 3 J/kg.
Peptides were selected for MS/MS using collision-induced dissociation operating mode with a normalized collision energy setting of 35%.
A sweeping collision energy setting of 37 ± 15 eV was applied to all precursor ions for collision-induced dissociation.
The most abundant 10 parent ions were selected for MS/MS using high-energy collision dissociation with a normalized collision energy setting of 45.
A sweeping collision energy setting of 35 ± 5 eV coupled with iTRAQ adjust rolling collision energy was applied to all precursor ions for collision-induced dissociation.
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