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DSE electrostatic interactions can be energetically significant and their modulation either by mutation or by varying solution conditions can have a major impact upon protein stability.
The stability and folding of proteins are modulated by energetically significant interactions in the denatured state that is in equilibrium with the native state.
Particularly in H2-rich ultramafic rock-hosted hydrothermal systems, anaerobic and aerobic hydrogenotrophy is more energetically significant than thiotrophy.
The program CaPTURE (Gallivan and Dougherty, 1999) identifies five of these contacts as energetically significant cation π interactions: F78 R82, R82 Y87, Y87 R150, K128 F146 and R145 Y149.
By contrast, we observe a substantial increase in the rate of slow inactivation with Ind in position 434, a result not compatible with the notion of an energetically significant cation-pi interaction at Trp434.
In both monocot and dicot AGPase SS equal numbers of energetically significant cation-pi interactions were observed which is parallel to the cation-pi interactions of the template protein.
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Thus energetically important hydrophobic contacts are those buried ones.
However, helping also involves significant costs in that it is energetically expensive [ 36, 37], and staying as a subordinate involves additional costs due to a strategic reduction of growth [ 28, 35, 38].
In an energetically congested start Adebayor's first significant act was to flex an arm into Louis Reed's face as he held him off on the halfway line, drawing a booking from the referee, Neil Swarbrick.
These experimental results clearly agree with recent theoretical calculations showing that the adsorption of Na+ ions on the surface of single layer graphene is energetically unfavourable unless that surface includes significant defects density.
However, this operation is energetically wasteful and would again require significant oxidative fluxes in the TCA.
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