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To this end IKOV genomic ends were obtained using circularization of the genomic RNA [ 22].
Uninterrupted insert sequences bounded by vector ends were obtained from 13 of 20 fosmid clones.
The 5′ and 3′ cDNA ends were obtained by touchdown PCR with the Advantage 2 PCR Enzyme System (Clontech), following the manufacturer's instructions.
The sequences of two additional Zorro3 5' ends were obtained by inverse PCR from C. albicans strain ATCC10261 [GenBank: DQ239689 and GenBank: DQ239690].
ESTs of 5′ and 3′ ends were obtained using the Sanger method with ABI 3730 sequencers following standard protocols for sequencing analysis.
After polyadenylation of 3′ ends of the genomic and complementary RNAs, the sequences of the segment ends were obtained by rapid amplification of cDNA ends.
Similar(49)
Theoretical and experimental results for flexural waves of a rectangular plate with free ends are obtained.
Polymers with thiol groups in side chains and chain ends are obtained by aminolysis.
A uniform linear line morphology with a smooth straight edge and symmetric ends was obtained at drop distance 1.35 ≤ y ≤ 1.60.
The analytical solutions of a beam with two simply supported ends are obtained as well as semi-analytical solutions are obtained for other end conditions using the differential quadrature method coupled with the state-space method.
The E7 gene with BamH1 and EcoR1 ends was obtained by PCR of the modified CRPV genome containing the Cla1 site and this allowed for placement of modified E7 genes into the wild type backbone.
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