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The beam ends were designed with standard, RBS, cover-plate, taper flange, and welded haunch respectively.
On the basis of four ω-secalin gene sequences cloned from Lankao 906 (a wheat cultivar with 1BL/1RS translocation) with putative transcription activity, a pair of primers with suitable restriction endonucleases added at their 5′ ends were designed to amplify the mature protein-coding regions of the four genes.
Briefly, PCR primers containing flanking attB sites (on their 5' ends) were designed to amplify each MPZ-MCS from human DNA.
Two types of adaptors, MseAD and SacAD, compatible with the MseI and SacI cohesive ends, were designed for RRL construction.
The 2 large flared ends were designed to protrude against the adjacent luminal walls with moderate pressure.
Two sets of consecutive mPing – specific primers respectively targeting the 5'- and 3'- mPing ends were designed and combined with HpaII/ MspI adaptor-primers (see Additional file 1).
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The bad guys have flip cars, sleek machines whose armor-plated front ends are designed so that when they strike another vehicle, it goes spinning through the air.
In this approach, an oligopeptide containing a cell adhesion domain (RGD) in the center and cysteine residues at both ends was designed.
In order to reduce the number of necessary PCRs, a pair of primers with truncated 3′ ends was designed in the present study.
Following HO cutting, the cassette generates two chromosomal fragments, whose ends are designed to invade each other via the YF2 and YF4 sequences.
Subsequently, primers including the 3′- and the 5′-ends were designed (see Table 1), which included the codon for the initiating methionine and a stop codon.
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