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To observe heart failure in the flies, a microscope slide with two electrodes on opposite ends was prepared [ 42].
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Poly ethylene oxide) (PEO) derivatives having both vinyl group and imidazolium salt structure on their ends were prepared and polymerized.
Autologous gracilis and semitendinosus tendons were harvested from their myotendinous junction, leaving their distal insertion intact: their proximal free ends were prepared with a Bunnel-type suture.
Oligomeric copolymers of dimethyl siloxane and 1,1,1-trifluoropropylmethyl sitoxane having functional amine groups at both ends were prepared and their microstructures were analysed by n.m.r.
Fluorescent PMMAs containing a phthalic anhydride (PA) group at the chain ends were prepared using the newly developed initiator 7 by atom transfer radical polymerization and subsequent pyrolysis.
The bone ends were prepared by removing all articular cartilage and preparing the appropriate surfaces for apposition.
Genomic DNA was extracted from females, and libraries with insert sizes of 310 and 630 bp (including the sequenced ends) were prepared.
In the EMSA 97 bp DNA fragments carrying Hsmar1 transposon ends were prepared by digesting pRC919 with XmaI and labeled at both ends using [α-P]dCTP and the Klenow enzyme.
Total RNA was extracted using TRIzol reagent (Life Technologies), and RNA-seq libraries for non-stranded paired ends were prepared by Illumina TruSeq Total RNA Sample Prep Kit according to its standard protocol unless specifically described below.
Well-defined poly vinyl alcohol) (PVA) macromonomer having c. two vinylbenzyl groups at the terminal end was prepared by means of free radical polymerization.
Duplex IR DNA containing the right inverted repeat sequence and with a 3 base overhang, corresponding to an excised transposon end, was prepared by annealing a 28-mer (5′-AAACGACATTTCATACTTGTACACCTGA-3′) and a complementary 25-mer (5′-GGTGTACAAGTATGAAATGTCGTTT-3′).
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