Hybridization material was generated through a random-priming amplification of poly [A]+ purified RNA using primers with a random sequence at the 3' end and a fixed motif at the 5' end that was optimized to generate strand-specific cDNA copies of full-length mRNA transcripts [ 32].
To this end, it was optimized for reusing the texture feature modules, giving, in this way, a more complete model for each object and becoming easier the object-discrimination process.
The design of this new chamber and of the front-end electronics was optimized to match the innovative features of the n_TOF facility, in particular the high instantaneous neutron flux and low background.
At the end, one mixture was optimized for a shelf time of at least 28 days without separation.
To this end, an extraction procedure was optimized using a Plackett Burman experimental design to determine the key conditions for optimal extraction of glucocorticoids from urine.
The effective spore end-concentration comprised 1011 spores/m2 and was optimized to cause high levels of infection whilst not causing too rapid mortality, in order to monitor possible interaction effects over time.
The gene structure was optimized according to the distribution of the reads, paired-end sequences and the annotation of reference genes.
The gene structure was optimized according to the distribution of the reads, information of paired-end and the annotation of reference gene.
The glucose and glutamine feeding rate was optimized to maximize the total amount of antibody produced at the end of the experiment.
By modelling of the hypothetical end member G Pr : Pr 0, using a temperature dependent contribution, the homogeneity range of CdPr was optimized according to the experimental data.
