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The compressive force applied at the ends was determined by deducting the prestress losses from the jacking force.
The sequence of the ssDNA ends was determined by similarity to phage PA6 after a PCR over the ends of the phage genome.
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The voltage sources position (one of the two line ends) is determined by the required current flow direction.
Later on, depending on the impact point of atmospheric discharges, different time instances, which the travelling waves require to arrive at their respective ends, are determined.
Iron concentrations in subcellular compartments (cell body, neurite outgrowths, and distal ends) were determined using PyMCA software [35] which enables to extract data from selected zones of the scanned area, and to fit corresponding X-ray fluorescence spectra.
SL RNA 3′ ends were determined essentially as described previously (Pettitt et al. 2008).
The sequences and structure of the genome ends were determined as previously described [ 40].
As a result, the sequences of 6 tomato PAL genes that differed in their noncoding 3' ends were determined.
ORF ends were determined as the coordinate of the first in phase stop codon encountered after the previously selected ATG start codon.
All CpG islands within 10 kb of the 5′ end of the sequence are located and their upstream (u) or downstream (d) position according to the 5′ ends is determined.
PCR products were cloned into the pCR®4-TOPO Vector (Invitrogen) and the DNA sequence of the 5'-prime-end was determined by dideoxy sequencing between 21 and 42 clones for each transgenic line.
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