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In order to reduce the number of necessary PCRs, a pair of primers with truncated 3′ ends was designed in the present study.
In this approach, an oligopeptide containing a cell adhesion domain (RGD) in the center and cysteine residues at both ends was designed.
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The bad guys have flip cars, sleek machines whose armor-plated front ends are designed so that when they strike another vehicle, it goes spinning through the air.
The beam ends were designed with standard, RBS, cover-plate, taper flange, and welded haunch respectively.
Briefly, PCR primers containing flanking attB sites (on their 5' ends) were designed to amplify each MPZ-MCS from human DNA.
The 2 large flared ends were designed to protrude against the adjacent luminal walls with moderate pressure.
Two types of adaptors, MseAD and SacAD, compatible with the MseI and SacI cohesive ends, were designed for RRL construction.
Two sets of consecutive mPing – specific primers respectively targeting the 5'- and 3'- mPing ends were designed and combined with HpaII/ MspI adaptor-primers (see Additional file 1).
Following HO cutting, the cassette generates two chromosomal fragments, whose ends are designed to invade each other via the YF2 and YF4 sequences.
The container, with a rope at each end, was designed so that opening it is easiest if two animals work together by pulling from either end.
Yet Obama's negotiating strategy, or lack of one, notwithstanding, the ACA in the end was designed to deliver health care using the existing model of private insurance using government subsidies for premiums.
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