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The 5' ends of the lux transcripts were mapped with the 5' RACE (Rapid Amplification of cDNA Ends) System Kit from Life Technologies, Inc., Rockville, USA. 1 to 5 μg of total RNA was used to synthesise cDNA from the 5' end of the lux mRNA with the gene-specific primer GSP1/luxA (CAACATAAGGATTCCC).
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"It is not a closed-end system".
hands-on setup of end-to-end system.
In the end, system characteristics and system error are summarized.
No-one has built a big end-to-end system yet".
Paper: "Interactive robogami: an end-to-end system for design of robots with ground locomotion".
PathDump, an end-to-end system for datacenter network monitoring and debugging.
Over the last two summers we have had two rehearsals of the end-to-end system.
A high-end system can cost as much as a flat-screen plasma television.
He was doing this in behalf of the telecom Orange, which makes the back-end system.
But both men hope to acquire a high-end system someday.
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