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All other combinations of the ends result in TMs that are responsive to the conformational changes associated with C-terminal modification.
Notably, although the cohesive ends generated by Xba I and Spe I are compatible, once religated together, they will give rise to a sequence which is no longer recognized by neither Xba I nor Spe I. Similarly, the cohesive ends generated by Sal I and Xho I are compatible, but religation of the ends result in a sequence which is no longer recognized by neither Sal I nor Xho I.
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The desired end result?
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The end result was workable.
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The end result was fantastic.
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