Sentence examples for ends of transcript from inspiring English sources

Next, the ends of transcript fragments are prepared to enable oligonucleotide adaptors to be ligated onto the ends.

Probe set location relative to the 5' and 3' ends of transcript reference sequences was calculated for DEC and INV probe sets that could be mapped to a single mRNA reference sequence (RefSeq) containing a terminal polyA sequence ≥ 10 nt.

This inconsistency may be due to RACE and MPSS tending to analyze sequences at the ends of transcript or due to the lack of sampling the appropriate tissues or conditions.

In contrast to the total RNA sample, the NRO sample showed an enrichment of reads near the 5′ ends of transcript models, with a read depth peak occurring approximately 50 bp downstream of the TSS, as has been observed in human and Drosophila cells (Core et al. 2008; Rasmussen and Lis 1993).

The CAGE method measures the expression levels of transcription starting sites by sequencing 5' ends of transcripts prepared through modifying their caps.

Specific sequencing of the 5' ends of transcripts was used for genome wide mapping of transcription start sites, allowing us to interrogate over 7000 promoters and 5′ untranslated regions.

This binding is enriched at the transcriptional start sites (TSSs), compared to the ends of transcripts (TxEnd), other gene body, or intergenic regions.

Therefore, we next investigated whether we could associate the H3K9me3 binding sites with 5' ends of transcripts.

The enriched transcripts were converted into cDNA by using the biotinylated primer based on the universal adaptor sequence at the 3' ends of transcripts.

Our approach comes from serial analysis of gene expression (SAGE) [7], which generates 21 bp tags from the 3' ends of transcripts.

CAGE is a high-throughput method to measure expression levels by counting large amounts of sequenced capped 5' ends of transcripts, termed CAGE tags [15], [16].