Sentence examples for ends of the inserts from inspiring English sources
The 5' ends of the inserts were sequenced with a single pass.
Sequencing reactions of the 5' ends of the inserts (standard T7 primer for libraries cloned in pBluescriptII SK+, primer 5'-CGAGCGCAGCGAGTCAGT-3' for libraries cloned in pDNR-LIB) were performed using BigDye terminator (Applied Biosystems) on two ABI 3730XL capillary system sequencers (Applied Biosystems).
Sequencing is carried out from both ends of the inserts using BigDye Terminators and ABI3730XL DNA sequencers.
The cDNA library was sequenced on the Illumina HiSeq™ 2000, and both ends of the inserts were sequenced.
Primer pairs were chosen from both ends of the inserts, which allows PCR to amplify the entire OHC cDNA insert.
Subsequently, 39,936 cDNA clones were randomly selected from the library and sequenced by a one-path method from both ends of the inserts.
E. coli recombinant clones were sequenced at both ends of the insert with flanking vector sequences as primers.
The protein was predicted to be a transposase in COG3335 (Expected value = 8e−14) and 27-nt imperfect inverted repeats were present at both ends of the insert.
We considered all TAR clones with a size >6.5 kb as positive and demonstrated that all clones with total sizes above 9 kb contained var genes as confirmed by sequencing of the 5' and 3' ends of the insert.
Both ends of the inserted fragments were sequenced.
