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Both reactions produced single products from the 5′ and 3′ ends of the class IIα gene, which were PCR-purified with Montage PCRμ96 Filter Plates (Millipore) and eluted in 20 μL ddH20 in preparation for sequencing.
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By the end of the class, my sons were smiling.
By the end of the class, none of the dogs seemed particularly eager to move.
"People come in somewhat reluctantly," he said at the end of the class.
I felt thoroughly worked out by the end of the class.
At the end of the class, she asked to look at my book and just smiled.
By the end of the class I am totally exhausted and just a little bit exhilarated.
At the end of the class, I was told that I "rocked it".
At the end of the class, everyone sits down to eat.
"It feels like a proper workout," business analyst Zoe gasped at the end of the class.
At the end of the class, there was a student left behind who was autistic.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com