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Immunohistochemistry showed well-formed actin fibers and β1 integrin staining at the ends of stress fibers.
The association of SYT strands with actin filaments diminished gradually toward the distal ends of stress fibers at the cell periphery, and as demonstrated by a lack of merged signal with paxillin, SYT did not extend into focal adhesions (Figure 5B).
These structures are distinct from focal adhesions, which are found at the ends of stress fibres in subconfluent endothelial cells.
This correlated with disruption of linear VE-cadherin localisation along cell cell junctions, particularly in regions at the ends of stress fibres.
Here we describe the distinct properties of endothelial cell-cell junctions that localize at the ends of stress fibres that we name discontinuous AJ.
In discontinuous AJ, VE-cadherin was found to align with the ends of stress fibres, some of which traversed the cell body form one side to the other.
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NMJs were identified at the distal ends of stressed and nonstressed axons, and the NMJ volume within the region of interest was quantitated using 3-dimensional images from confocal stacks.
In any case, Friday's results did not mark the end of stress tests; in fact, they are only the beginning.
The stress-strain curves can be defined into four zones with the onset and the end of stress plateau, and the strain hardening point as the zone boundaries.
DRonset corresponded to the phyllochron computed from the period of stress onset to the end of stress, both for stressed and its corresponding well watered plant.
Genotype differences for the number of days during the stress period was tested with a one way ANOVA on the number of days from stress onset (FTSW 1) and the end of stress (FTSW0.2).
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