Sentence examples for ends of sequencing from inspiring English sources

Previously specialized algorithms developed for DGF have identified hundreds of TF binding sites at genome-wide resolution, by comparing the depth of DNase I digestion at TF binding sites to adjacent open chromatin and taking into account only raw counts of 5′ ends of sequencing tags [ 19, 78, 83, 128, 146– 146].

Several technical comments on that study [ 19- 21] pointed out that the mismatches of RNA-seq reads to the reference genome are almost exclusively at the ends of sequencing reads.

To help users account for this, RNA-Rocket provides access to the FastQC tool (http://www.bioinformatics.babraham.ac.uk/projects/fastqc) for determining the quality profile of sample reads, as well as Sickle (https://github.com/najoshi/sickle) and Trimmomatic (Bolger et al., 2014), which can be used to automatically trim off low quality base calls from the ends of sequencing reads.

Obviously, the color code bias problem is most severe near the end of sequencing.

Furthermore, because it is a local alignment approach, it was expected to trim the ends of sequences that were sufficiently different from their template.

The ends of sequence identity were determined based on BLASTN results.

Autapomorphic insertions and ends of sequences were removed from each alignment.

An in-house program called QUALSCREEN was used to quality clip the ends of sequence traces.

Prior to alignment, all sequences were trimmed to remove low-quality bases at the 5′ and 3′ ends of sequences.

We wanted to assess the correlation between the locations of the 5' ends of sequences deriving from opposite strands.

Allowing for the possibility of low quality reads at the ends of sequences, we evaluated read quality using Galaxy.