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In order to minimize the mining of technical errors we defined a stringent threshold for alignment quality (expect≤1E-21) and the low quality ends of alignments were discarded.
The repeat sequences were reinserted into the pairwise alignments, the ends of alignments were trimmed, and global alignments were generated (see reference [ 66] for details).
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k-mers in a read appearing in the de Bruijn graph is considering as potential aligned position and IDBA-Tran will try to extend both ends of alignment considering substitution error only.
Sequences of lipoxygenases in the six Lx regions of soybean and Medicago were compiled and aligned using ClustalX and sequence overhang at the 5'- and 3'-end of alignments were removed.
The second characteristic of false positives is that some tend to occur towards the end of alignments where there is some uncertainty about the pairing of read and genomic sequence.
In contrast to conventional methods, the signature method utilizes information present in the sequences that may not be analyzable with conventional alignments, such as additional sequences at the beginning or the end of alignments.
Alignment quality is especially problematic in the end regions of the intraspecies alignments, because (1) the ends of local alignments are generally defined by falling scores, and (2) the paralogs used by CHAP are entire local alignments, though their corresponding orthologs are usually excerpts from longer alignments.
We chose unambiguously aligned regions, removing the ends of the alignments, and excluded CpG sites from the alignments.
For the selected alignments, all unaligned bases in 3′ ends of local alignments were regarded as non-templated additions.
In these alignments, the proportion of mismatches was highest at the ends of these alignments where there are typically lower numbers of sequencing reads (Fig. 3).> -wrap-foot> Summary of BLASTN searches of the transcriptome using published sequences from five protein-coding genes as queries.
This situation is common and is due to uncertainty about the ends of local alignments.
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CEO of Professional Science Editing for Scientists @ prosciediting.com