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Incorrectly ligated fragments containing B adaptors at both ends failed to bind to beads initially, allowing their removal.
However, because (1) some ends failed to obtain nucleotide sequences; (2) some end sequences were unable to design suitable primerpairs; (3) some end primers screened BAC library unsuccessfully, two BAC clones (692B2 and 1262B6) underwent shotgun sequencing to design new primers adjacent to ends.
Similar(57)
His major-label deal, now ended, failed to find him a substantial audience.
In 2010 the rainbow bridge that leads the gods into Valhalla at the end failed to materialize.
Even hopes stirred by a previously unreleased recording by Arthur Rubinstein in the end failed to do the trick.
The critics say the swap, completed months after the first quarter ended, failed to prove that there was an active or liquid market for long-term power deals, a test the auditors were seeking to establish.
Russia became increasingly frustrated and, in the end, failed to get the result they needed to go top of Group F. The night belonged to Northern Ireland and manager O'Neill. .
The comedy "John Dies at the End" failed to provide its promised laughs, while "Parker" fizzled out following a riveting beginning.
After a hit on Chicago defenseman Johnny Oduya bloodied the Blackhawk's face and got Ducks forward Kyle Palmieri ejected, reporters looking to speak to Palmieri at game's end failed to find him.
Although the Third Crusade, in the end, failed to retake Jerusalem, a three year truce, the Treaty of Jaffa, was eventually negotiated with Saladin - Christian pilgrims from the west would once again be allowed to visit Jerusalem.
He felt that the solid plot and the unambiguous storytelling of The Immortals of Meluha was missing in The Secret of the Nagas, whose ending failed to live up the curiosity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com