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{According to NEB, decreasing temperataure and incubation time to 12°C for 15 minutes reduces the occurrence of recessed ends caused by the 3' → 5' exonuclease activity of T4 DNA polymerase}.
Moreover, we modulated protein stability throughout the entire trajectory using overexpression of GroEL/ES to avoid evolutionary dead ends caused by stability bottlenecks (Socha and Tokuriki, 2013; Wyganowski et al., 2013).
However, the majority of these L1 elements are non-functional relics due to severe truncation at their 5' ends caused by aborted TPRT events and accumulation of deleterious mutations either over time or as a result of unfaithful RT activity [ 2, 20- 23].
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In agreement with this hypothesis, progerin expression destabilizes chromosome ends, causing DNA damage and premature senescence (Benson et al., 2010; Wood et al., 2014).
Split ends cause your hair to be less healthy.
Also, don't iron it everyday; this will slowly burn the hair and fry its ends, causing broken and damaged hair.
"That little wrinkle at the end caused a lot of anxiety," Mr. Nickles said.
Why have the photographs and films of Gaddafi's end caused so much fuss and bother?
It's as if the way something ended caused her to reconsider everything that came before.
"It felt like a media phenomenon that in the end caused relatively few deaths.
The big public debts of many countries when the second world war ended caused huge anxieties, but the burden diminished rapidly thanks to fast economic growth.
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