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The capillaries pass through two opposing channels and their injection ends are located opposite one another at a distance of approx. 0.5 mm in the centre of the crossing point.
The actin filaments in the stereocilium core are uniformly polarized such that their barbed (plus, polymerizing) ends are located at stereocilia tips.
We developed a program to be able to analyze this great volume of sequencing data, named GenoSeqGrapher V 1.0 (Taboada, B. et al, manuscript in preparation) that graphically displays each sequence below their corresponding position in the E coli genome, so that it is very easy to detect where the cDNA ends are located in the genome (Figure 9).
The S. cerevisiae genome was annotated to have 1561 convergent, 1569 divergent, and 2906 tandem intergenic regions whose ends are located more than 400 bases from chromosome telomeres.
The elongating tubules move more rapidly forward so that by the end of embryogenesis, the distal tubule ends are located both more anterior (in A1) and further ventral in the body cavity.
Importantly, we found that while chromosomes move inwards in monopolar anaphase, the ring structures could be observed out towards the periphery of the monopolar spindle, where the plus ends are located.
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Minus ends are locating targets with an exactitude comparable to that which has been demonstrated for plus ends in certain cell types.
However, I had hoped to hear where the big breakthroughs would come, and also where the dead ends were located.
Suitable nanowire junctions with electrodes at the ends were located using optical microscopy and later probed with an Agilent 4155C Semiconductor Analyzer as the current forcing device.
We quantified the expression of bidirectional loci for each strand and 200 bp flanking window in each sample separately by counting the CAGE tags whose 5′ ends were located within these windows.
For the 320 predicted rho-independent terminators we found that the majority of transcript ends were located within stem 1 of the predicted rho-independent terminator and the minority within the loop, stem 2, or T-tail (72.2% within stem 1, 18.4% within the loop, 7.5% within stem 2, and 1.9% within the T-tail; Figure 8, Additional file 1: Table S9).
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