Sentence examples similar to endogenous restrictions from inspiring English sources

The studies above in permissive cells involve expression of CAML or tetherin from expression plasmids with strong promoters, and may not reflect the endogenous restriction found in restrictive human cells.

Portions of the 5' untranslated region (UTR) of pCAGGS-MCS downstream of the CAG promoter were progressively truncated using endogenous restriction enzyme sites within the UTR sequence.

An endogenous restriction site close to the C-terminal end (AgeI) was used to clone in the CFP (Table S1), resulting in the plasmid pTOPO AtCRT3-CFP.

Five endogenous restriction sites in rNav1.2α ORF were used to excise DNA fragments coding for the four channel domains (DI: XhoI/XmaI, DII: XmaI/BglII, DIII: BglII/BstEII, DIV: BstEII/PacI).

Mutations were made using PCR, and the mutated fragment was subcloned back into pCS2-Nrp1 using endogenous restriction sites.

Briefly, we digested the PCR product using one or two endogenous restriction enzymes (TaqαI, RsaI, BstBI, or BstUI).

Site-specific ZFN function was verified by a genotyping strategy wherein introduction of mutagenic lesions at the target site leads to loss of a unique endogenous restriction site.

The low probability of the presence of endogenous restriction sites decreases the risk of genome fractionation by the meganuclease upon expression.

Plasmid transformation in Cpa has been shown previously using the heterologous M.FnuDII methyltransferase (5′-CmGCG-3′) to evade the endogenous restriction enzyme [ 11].

Endogenous restriction enzymes BamHI and XbaI were used to digest the plasmid (pNIM-003), to check the correction of the fragment size.

The transformation efficiency for the positive control (pAM5 plasmid DNA isolated from B. longum subsp. longum NCIMB 8809) is still 10-fold higher than that achieved for plasmid DNA isolated from the E. coli EC101 derivative harbouring plasmid pNZEM-M.blmncII, which suggests that the latter plasmid is not fully protected against the endogenous restriction activity of NCIMB 8809.