Sentence examples for endogenous precipitation from inspiring English sources

Due to its similarity to endogenous bone, the CERAMENT™ implant triggers an endogenous precipitation of hydroxyapatite on its surface which prevents passive resorption.

To obtain evidence that the p53-inducing activity of the scaffold-presented peptides resulted from an interaction between the CI2-presented peptides with endogenous Mdm2, co-precipitation experiments were performed.

(E) Co-IP using anti-Sp1 antibody or IgG were performed in 293T cells with or without H2O2 exposure, and precipitation of endogenous SENP3 was determined by IB with anti-SENP3 antibody.

Chromatin immunoprecipitations were performed using 2 µg Invitrogen anti-GFP A11122 antibody overnight at 4 C. We chose to use the anti-GFP antibody rather than anti-MeCP2 to avoid precipitation of endogenous MeCP2 in the transfected mouse cells.

Free and total insulin were measured by radioimmunoassay after precipitation of endogenous antibodies with polyethylene glycol.

We then confirmed that p110α indeed specifically binds to BMPRII by precipitation of endogenous p110α which co-immunoprecipitated BMPRII in a BMP2-dependent manner.

For precipitation of endogenous HIF-1 α, Cezanne-depleted and control cells were treated with 20 μM MG132 for 2 h (6-well plate) and subsequently lysed in 100 μl lysis buffer per well (50 mM Tris HCl (pH 7.5), 150 mM NaCl, 1% (v/v) IGEPAL® CA-630, 2 mM EDTA, 1 mM dithiothreitol, 100 mM NaF and 1 tablet/10 ml cOmplete, Mini, EDTA-free protease inhibitors (Roche)).

While M2A8 (Fig 1A, fusion of exon 2 of MALT1 to exon 8 of API2) did not bind BCL10, the inclusion of one or two Ig-like domains of MALT1 in the MALT1-API2 fusion variants M4A8 and M7A8/M8A8, respectively (Fig 1A, fusion of exon 4, 7 and 8 of MALT1, respectively, to exon 8 of API2) resulted in co-precipitation of endogenous BCL10, similarly as for MALT1 (Fig 2A).

As shown in Figure 3A, co-precipitation between endogenous TLR4 and Siglec-E was substantially reduced after LPS stimulation.

Co-precipitation of endogenous TDP-43 with each these Flag-proteins was then evaluated by western blot.

In Huh7 cells expressing HBx-GFP, immunoprecipitation of HBx using GFP antibody resulted in a co-precipitation of endogenous Keap1 and p62.