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First, MeP retrovirus vectors can modify NSC that subsequently differentiate into cells that express MeCP2 in the endogenous pattern.
To overcome this problem we used electroporation conditions for transfecting scattered cells with high levels of Delta-1 expression, emulating the endogenous pattern of expression.
This might not completely describe the M6 endogenous pattern evidenced by the enhancer trap GFP-M6, which in turn reflects the pattern of isoforms driven by P1 (and not P2).
We first examined the endogenous pattern of Snail1 expression in the perichondral area of mouse embryos throughout fetal development.
To evaluate the potential therapeutic effect of SMN-specific U1s in SMA, we transfected sm17 and sm21 in HEK293 cells and evaluated the endogenous pattern of splicing.
The observed 30.4% ciliary localization is likely to be an underestimation, as steric interference from the C-terminal GFP tag may affect the endogenous pattern of protein localization.
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Toll Like Receptors (TLRs) are receptors of innate immunity and recognize diverse exogenous and endogenous patterns.
The forced appropriation of farmlands unsettles endogenous patterns of mixed-use development or "rural-urbanization" which have developed in these zones since the đổi mới, and which are characterized by the combination of small-scale cottage industries with mixed employment, and commuting into the cities for jobs and trade.
For example, behavioral economics [1] builds on cognitive and emotional models of agents, neuroeconomics [2] addresses the neurobiological basis of valuation of choices, and evolutionary economics [3] studies economies as a complex evolutionary system, composed of agents that adapt to endogenous patterns out of equilibrium regions.
In fact, early results from GFP-tagged coding regions using bacterial recombineering in fosmid clones look very promising in terms of recapitulating endogenous patterns of transcription factor expression and binding [1], [2].
Although Nematostella is a member of the anthozoan cnidarians (known for producing an abundance of diverse fluorescent proteins (FPs)), endogenous patterns of Nematostella fluorescence have not been described and putative FPs encoded by the genome have not been characterized.
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