Sentence examples for endogenous p62 from inspiring English sources

To assess endogenous p62 localization, SH-SY5Y cells with inducible expression of miRNA targeting mortalin were seeded in 96-well plates (10 000 cells per well) and grown in tetracycline-containing medium for 72 h followed by a 3-h treatment with control medium (CM) or EBSS in the presence or absence of BafA1 include concentration of BafA (200nM).

Co-immunoprecipitation analysis showed that endogenous p62 interacts with endogenous parkin (Fig. 4A and 4B).

Ectopically expressed GFP-parkin, but not GFP itself, could interact with endogenous p62 (Fig. 4D).

Further, ectopically expressed ALS2 was colocalized with either endogenous p62 (Figure 7C) or EGFP-LC3 (Figure 7D) onto the vesicular compartments in primary hippocampal neurons.

Distribution of ectopically expressed ALS2 and EGFP-LC3 extensively overlapped with endogenous p62 in vesicular compartments in fibroblasts (Figure 7A and 7B).

Because p62/SQSTM1 is a selective substrate of autophagy and, hence, monitoring endogenous p62 protein levels indirectly measures autophagic degradation, we concurrently monitored how acquisition of breast cancer auto-resistance to Tzb affected cellular distribution of both p62 and LC3 (Figure 5).

We found that anti-LRRK2 pulled down endogenous Rip2 (Fig. 6E), indicating an association of endogenous LRRK2 and Rip2.

It is encoded on the endogenous 84-kb plasmid pBS32.

Endogenous Fe2+ concentrations (100 μM) inhibit cat B only marginally.

The turnover of endogenous Robo1 was determined by Western blot.

In addition, we employed shRNA to suppress endogenous UHRF2.