We have shown that if several 2D-gel spots are being processed, CK's represented in less than 5%percentt of the spots are very likely to be endogenous identifications (CK's 3, 4, 7, 8, 11, 12, 13, 15, 17, 18, 19, 20 and 23).
Sparse use of variable modifications is disadvantageous to endogenous peptide identification as many of these peptides are known to be modified [ 26].
For the endogenous peptide identification, methionine oxidation was set as a variable modification, with a tolerance of 0.1 Da for both the precursor and fragment ions.
DOI: http://dx.doi.org/10.7554/eLife.05544.007 While we know that ANGPTLs has direct effects on several mammalian cell types in culture (Kubota et al., 2005) but because they are secreted factors, the lack of their endogenous receptor identification in zebrafish limits our ability to assess which endogenous cell population they act upon.
Collision energy for each metabolite was optimized for maximum fragmentation efficiency, and identical collision energies were used for each chemical standard and the endogenous metabolite for identification.
For an endogenous construct, the identification condition requires specifying the construct's formative indicators as endogenous variables [ 85]. cThe three "EMR functional coverage" variables and the three "extended EMR use" variables are operationalized through "index" rather than "scale" measures [ 86].
HPRT was used as endogenous control (RTPrimerDB identification no. 3600) and the calculation of qPCRs was performed as described [ 41]; data were calibrated to the untreated state of the respective genotype.
The identification of endogenous avian leukosis virus (ALV) and endogenous avian retrovirus (EAV) in chick cell-derived measles and mumps vaccines in current use has raised concern about transmission of these retroviruses to vaccine recipients.
The identification of endogenous cis-regulatory DNA elements (CREs) responsive to endogenous and environmental cues is important for studying gene regulation and for biotechnological applications but is labor and time intensive.
Furthermore, the present active CYP130-fusion protein may facilitate the identification of endogenous substrates from Mtb.
