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A genetically engineered cardiac TnC mutant labeled at Cys-84 with tetramethylrhodamine-5-iodoacetamide dihydroiodide was passively exchanged for the endogenous form in skinned guinea pig trabeculae.
This chapter will begin with a discussion on distraction osteogenesis, an endogenous form of skeletal tissue engineering, and the molecular lessons gleaned from it.
Results show that combustion chamber (CC) concentrates most of the exergy destruction (more than 62%), dominantly in unavoidable endogenous form which is decreased by 11.89% and 13.12% while the avoidable endogenous exergy destruction increase and is multiplied by the factors of 1.3 and 8.6 with increasing TIT and rc, respectively.
Mature hFXN81 210 is the main endogenous form of frataxin in human tissues and cell lines [24].
The endogenous Armadillo protein levels tended to be inversely related to those of the overexpressed construct, suggesting that the endogenous form was excluded from the AJ.
Since all three N-terminal deletion mutants produce similar effects at the AJ, it is likely due to their increased stability relative to the endogenous form, mediated in large part by their escape from Shaggy/GSK3 regulation.
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The interaction of both proteins was further confirmed in their endogenous forms by co-immunoprecipitation assay.
Reports have shown that exogenous and endogenous forms of NO induce apoptosis in MKs.
However, the endogenous forms of this protein have not been characterized so far.
The Is[lin-28 gfp] straIs[lin-28 gfpthe endogenous forms of LIN-28 and the shifted fusion protein.
The following six constructs (five exogenous and one endogenous) formed the latent variables (measurement part) in our statistical models.
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